R+R Medicinals 500mg Organic Full Spectrum Pet Tincture

Certificate of Analysis

HEMP QUALITY ASSURANCE TEST

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Sample Name:

R+R Medicinals 500mg Organic Full Spectrum Pet Tincture

Infused, Liquid Edible

Total THC 22.770 mg/unit

Total CBD 515.370 mg/unit

Sum Cannabinoids 592.680 mg/unit

Total Cannabinoids 592.680 mg/unit

Total Terpenoids 0.0827%

Top 3 Terpenoids

β-Caryophyllene 0.0358%
α-Bisabolol 0.0169%
α-Humulene 0.0148%

Sample Information

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Sample Details

Sample ID:210729U001

Batch Number:0706

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Cannabinoid Analysis - Summary

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Total THC: 22.770 mg/unit

Total CBD: 515.370 mg/unit

Sum of Cannabinoids: 592.680 mg/unit

Total Cannabinoids: 592.680 mg/unit

Density:0.923 g/mL

Total THC/CBD is calculated using the following formulas to take into account the loss of a carboxyl group during the decarboxylation step:
Total THC = Δ⁹-THC + (THCa (0.877))
Total CBD = CBD + (CBDa (0.877))

Sum of Cannabinoids = Δ⁹-THC + THCa + CBD + CBDa + CBG + CBGa + THCV + THCVa + CBC + CBCa + CBDV + CBDVa + Δ⁸-THC + CBL + CBN

Total Cannabinoids = (Δ⁹-THC+0.877*THCa) + (CBD+0.877*CBDa) + (CBG+0.877*CBGa) + (THCV+0.877*THCVa) + (CBC+0.877*CBCa) + (CBDV+0.877*CBDVa) + Δ⁸-THC + CBL + CBN

Why are Sum of Cannabinoids and Total Cannabinoids calculated separately?

Sum of Cannabinoids: The concentration of each individual cannabinoid added together as they are, without accounting for decarboxylation. This measurement is of particular importance for certain cannabis products that are not intended to be heated prior to application or consumption.

Total Cannabinoids: The concentration of each acidic cannabinoid, multiplied by 0.877 to account for the loss of molecular mass that happens as it is converted to a neutral cannabinoid when heated. Then this calculated concentration is added to the concentrations of the corresponding neutral cannabinoid already present. This corrected data is of particular importance to both producers and consumers of products intended to be heated prior to application or consumption.

Terpenoid Analysis - Summary

39 tested, Top 3 Highlighted

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Total Terpenoids: 0.0827%

β-Caryophyllene (0.0358%)

α-Bisabolol (0.0169%)

α-Humulene (0.0148%)

Safety Analysis - Summary

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Δ⁹-THC per Unit: Pass

Pesticides: ND

Mycotoxins: ND

Residual Solvents: ND

Heavy Metals: ND

Microbiology (PCR): ND

Microbiology (Plating): ND

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Cannabinoid Analysis

Tested

Tested by high-performance liquid chromatography with diode-array detection (HPLC-DAD).

Method: QSP 1157 - Analysis of Cannabinoids by HPLC-DAD

Summary

Total THC: 22.770 mg/unit

(Δ⁹-THC+0.877*THCa)

Total CBD: 515.370 mg/unit

(CBD+0.877*CBDa)

Total Cannabinoids: 592.680 mg/unit

Total CBG: 12.630 mg/unit

Total CBG (CBG+0.877*CBGa)

Total THCV: ND

Total THCV (THCV+0.877*THCVa)

Total CBC: 36.360 mg/unit

Total CBC (CBC+0.877*CBCa)

Total CBDV: 4.290 mg/unit

Total CBDV (CBDV+0.877*CBDVa)

Learn more

The cannabis plant contains dozens of active compounds called cannabinoids. These compounds are the primary contributors to the psychoactive effects of cannabis.

Cannabinoid testing determines the potency of a sample to aid in dosage considerations.

Cannabinoid Test Results

07/29/2021

Result Views

Table Pie Chart

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Compound	LOD/LOQ (mg/mL)	Measurement Uncertainty (mg/mL)	Result (mg/mL)	Result (%)
Cannabidiol (CBD)	0.004 / 0.011	±0.8229	17.179	1.8612
Cannabichromene (CBC)	0.003 / 0.010	±0.0502	1.212	0.1313
Δ9 Tetrahydrocannabinol (Δ9THC)	0.002 / 0.014	±0.0535	0.759	0.0822
Cannabigerol (CBG)	0.002 / 0.006	±0.0262	0.421	0.0456
Cannabidivarin (CBDV)	0.002 / 0.012	±0.0075	0.143	0.0155
Cannabicyclol (CBL)	0.003 / 0.010	±0.0017	0.035	0.0038
Cannabinol (CBN)	0.001 / 0.007	±0.0003	0.007	0.0008
Cannabichromenic Acid (CBCa)	0.001 / 0.015	N/A	ND	ND
Cannabidiolic Acid (CBDa)	0.001 / 0.026	N/A	ND	ND
Cannabigerolic Acid (CBGa)	0.002 / 0.007	N/A	ND	ND
Tetrahydrocannabivarin (THCV)	0.002 / 0.012	N/A	ND	ND
Tetrahydrocannabinolic Acid (THCa)	0.001 / 0.005	N/A	ND	ND
Cannabidivarinic Acid (CBDVa)	0.001 / 0.018	N/A	ND	ND
Tetrahydrocannabivarinic Acid (THCVa)	0.002 / 0.019	N/A	ND	ND
∆8 Tetrahydrocannabinol (Δ8THC)	0.01 / 0.02	N/A	ND	ND
SUM OF CANNABINOIDS			19.756 mg/mL	2.1404%

Unit Mass:

30 milliliters

Serving Size:

1 milliliters

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Δ⁹-THC per Unit	112 per-package limit	22.770 mg/unit	Pass
Δ⁹-THC per Serving		0.759 mg/serving
Total THC per Unit		22.770 mg/unit
Total THC Per Serving		0.759 mg/serving
CBD per Unit		515.370 mg/unit
CBD per Serving		17.179 mg/serving
Total CBD per Unit		515.370 mg/unit
Total CBD per Serving		17.179 mg/serving
Sum of Cannabinoids per Unit		592.680 mg/unit
Sum of Cannabinoids per Serving		19.756 mg/serving
Total Cannabinoids per Unit		592.680 mg/unit
Total Cannabinoids per Serving		19.756 mg/serving

Density Test Result

0.923 g/mL Tested 07/29/2021 Method: QSP 7870 - Sample Preparation

Learn more

The cannabis plant contains dozens of active compounds called cannabinoids. These compounds are the primary contributors to the psychoactive effects of cannabis.

Cannabinoid testing determines the potency of a sample to aid in dosage considerations.

Terpenoid Analysis

Tested

Terpene analysis utilizing gas chromatography-flame ionization detection (GC-FID).

Method: QSP 1192 - Analysis of Terpenoids by GC-FID

Summary

Total Terpenoids (mg/g): 0.827 mg/g

Total Terpenoids (%): 0.0827%

Dominant Terpenoids

Below are this sample's 3 most abundant terpenoids by volume.

β-Caryophyllene 0.0358%
A sesquiterpene with a fragrance that can be described as spicy, woody, dry, dusty and mildly sweet. It was one of the first organic compounds to fully synthesized in a laboratory and plays a role in the endocannabinoid system as it is a functional CB² receptor agonist. Found in black pepper, clove, hops, rosemary, black-jack, perilla, spicebush, Indian pennywort, celery, frankincense, vitex, parsley, marigold, tamarind...etc.

α-Bisabolol 0.0169%
A sesquiterpene alcohol with a fragrance that can be described as floral, peppery, sweet and clean. Found in chamomile, figwort, yarrow, skullcaps, lavender, ironwort, germander...etc.

α-Humulene 0.0148%
Also known as α-caryophyllene, it is an isomer of the sesquiterpene β-Caryophyllene which frequently occurs in nature with many aromatic plants across the globe. It has a fragrance that can be described as earthy or musky with spicy undertones. Found in hops, forskohlii, skullcaps, basil, nutmeg, cloves, sage, cotton, tamarind, black pepper, guava, Scotch pine...etc.

Learn more

Terpenoid analysis is crucial for differentiating between strains of cannabis, as terpenoids have a major influence on the medical and psychological effects of a plant. The relationship between cannabinoids and terpeneoids is known as the "entourage effect."

Terpenoid Test Results

07/29/2021

Result Views

Table Bar Graph

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Compound	LOD/LOQ (mg/g)	Measurement Uncertainty (mg/g)	Result (mg/g)	Result (%)
β-Caryophyllene	0.004 / 0.012	±0.0127	0.358	0.0358
α-Bisabolol	0.008 / 0.026	±0.0090	0.169	0.0169
α-Humulene	0.009 / 0.029	±0.0048	0.148	0.0148
Guaiol	0.009 / 0.030	±0.0043	0.092	0.0092
Caryophyllene Oxide	0.010 / 0.033	±0.0028	0.060	0.0060
Borneol	0.005 / 0.016	N/A	<LOQ	<LOQ
Fenchol	0.010 / 0.034	N/A	<LOQ	<LOQ
Myrcene	0.008 / 0.025	N/A	<LOQ	<LOQ
Limonene	0.005 / 0.016	N/A	<LOQ	<LOQ
Linalool	0.009 / 0.032	N/A	<LOQ	<LOQ
Nerolidol	0.009 / 0.028	N/A	<LOQ	<LOQ
Terpineol	0.016 / 0.055	N/A	<LOQ	<LOQ
Valencene	0.009 / 0.030	N/A	<LOQ	<LOQ
Eucalyptol	0.006 / 0.018	N/A	<LOQ	<LOQ
trans-β-Farnesene	0.008 / 0.025	N/A	<LOQ	<LOQ
Nerol	0.003 / 0.011	N/A	ND	ND
Cedrol	0.008 / 0.027	N/A	ND	ND
Camphor	0.006 / 0.019	N/A	ND	ND
Menthol	0.008 / 0.025	N/A	ND	ND
Camphene	0.005 / 0.015	N/A	ND	ND
Fenchone	0.009 / 0.028	N/A	ND	ND
Geraniol	0.002 / 0.007	N/A	ND	ND
Pulegone	0.003 / 0.011	N/A	ND	ND
Sabinene	0.004 / 0.014	N/A	ND	ND
p-Cymene	0.005 / 0.016	N/A	ND	ND
Isoborneol	0.004 / 0.012	N/A	ND	ND
Isopulegol	0.005 / 0.016	N/A	ND	ND
Citronellol	0.003 / 0.010	N/A	ND	ND
Terpinolene	0.008 / 0.026	N/A	ND	ND
β-Pinene	0.004 / 0.014	N/A	ND	ND
α-Pinene	0.005 / 0.017	N/A	ND	ND
β-Ocimene	0.011 / 0.038	N/A	ND	ND
Δ³-Carene	0.005 / 0.018	N/A	ND	ND
α-Cedrene	0.005 / 0.016	N/A	ND	ND
Geranyl Acetate	0.004 / 0.014	N/A	ND	ND
α-Terpinene	0.005 / 0.017	N/A	ND	ND
γ-Terpinene	0.006 / 0.018	N/A	ND	ND
Sabinene Hydrate	0.006 / 0.022	N/A	ND	ND
α-Phellandrene	0.006 / 0.020	N/A	ND	ND
TOTAL			0.827 mg/g	0.0827%

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Pesticide Analysis

Pesticide and plant growth regulator analysis utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS) or gas chromatography-mass spectrometry (GC-MS).

*GC-MS utilized where indicated.

Method: QSP 1212 - Analysis of Pesticides and Mycotoxins by LC-MS or QSP 1213 - Analysis of Pesticides by GC-MS

Pesticide Test Results

07/31/2021

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)
Aldicarb	0.03 / 0.08	≥ LOD	N/A	ND
Carbofuran	0.02 / 0.05	≥ LOD	N/A	ND
Chlordane*	0.03 / 0.08	≥ LOD	N/A	ND
Chlorfenapyr*	0.03 / 0.10	≥ LOD	N/A	ND
Chlorpyrifos	0.02 / 0.06	≥ LOD	N/A	ND
Coumaphos	0.02 / 0.07	≥ LOD	N/A	ND
Daminozide	0.02 / 0.07	≥ LOD	N/A	ND
Dichlorvos (DDVP)	0.03 / 0.09	≥ LOD	N/A	ND
Dimethoate	0.03 / 0.08	≥ LOD	N/A	ND
Ethoprophos	0.03 / 0.10	≥ LOD	N/A	ND
Etofenprox	0.02 / 0.06	≥ LOD	N/A	ND
Fenoxycarb	0.03 / 0.08	≥ LOD	N/A	ND
Fipronil	0.03 / 0.08	≥ LOD	N/A	ND
Imazalil	0.02 / 0.06	≥ LOD	N/A	ND
Methiocarb	0.02 / 0.07	≥ LOD	N/A	ND
Mevinphos	0.03 / 0.09	≥ LOD	N/A	ND
Paclobutrazol	0.02 / 0.05	≥ LOD	N/A	ND
Parathion-methyl	0.03 / 0.10	≥ LOD	N/A	ND
Propoxur	0.03 / 0.09	≥ LOD	N/A	ND
Spiroxamine	0.03 / 0.08	≥ LOD	N/A	ND
Thiacloprid	0.03 / 0.10	≥ LOD	N/A	ND
Abamectin	0.03 / 0.10	0.3	N/A	ND
Acephate	0.02 / 0.07	5	N/A	ND
Acequinocyl	0.02 / 0.07	4	N/A	ND
Acetamiprid	0.02 / 0.05	5	N/A	ND
Azoxystrobin	0.02 / 0.07	40	N/A	ND
Bifenazate	0.01 / 0.04	5	N/A	ND
Bifenthrin	0.02 / 0.05	0.5	N/A	ND
Boscalid	0.03 / 0.09	10	N/A	ND
Captan	0.19 / 0.57	5	N/A	ND
Carbaryl	0.02 / 0.06	0.5	N/A	ND
Chlorantraniliprole	0.04 / 0.12	40	N/A	ND
Clofentezine	0.03 / 0.09	0.5	N/A	ND
Cyfluthrin	0.12 / 0.38	1	N/A	ND
Cypermethrin	0.11 / 0.32	1	N/A	ND
Diazinon	0.02 / 0.05	0.2	N/A	ND
Dimethomorph	0.03 / 0.09	20	N/A	ND
Etoxazole	0.02 / 0.06	1.5	N/A	ND
Fenhexamid	0.03 / 0.09	10	N/A	ND
Fenpyroximate	0.02 / 0.06	2	N/A	ND
Flonicamid	0.03 / 0.10	2	N/A	ND
Fludioxonil	0.03 / 0.10	30	N/A	ND
Hexythiazox	0.02 / 0.07	2	N/A	ND
Imidacloprid	0.04 / 0.11	3	N/A	ND
Kresoxim-methyl	0.02 / 0.07	1	N/A	ND
Malathion	0.03 / 0.09	5	N/A	ND
Metalaxyl	0.02 / 0.07	15	N/A	ND
Methomyl	0.03 / 0.10	0.1	N/A	ND
Myclobutanil	0.03 / 0.09	9	N/A	ND
Naled	0.02 / 0.07	0.5	N/A	ND
Oxamyl	0.04 / 0.11	0.2	N/A	ND
Pentachloronitrobenzene (Quintozene)*	0.03 / 0.09	0.2	N/A	ND
Permethrin	0.04 / 0.12	20	N/A	ND
Phosmet	0.03 / 0.10	0.2	N/A	ND
Piperonyl Butoxide	0.02 / 0.07	8	N/A	ND
Prallethrin	0.03 / 0.08	0.4	N/A	ND
Propiconazole	0.02 / 0.07	20	N/A	ND
Pyrethrins	0.04 / 0.12	1	N/A	ND
Pyridaben	0.02 / 0.07	3	N/A	ND
Spinetoram	0.02 / 0.07	3	N/A	ND
Spinosad	0.02 / 0.07	3	N/A	ND
Spiromesifen	0.02 / 0.05	12	N/A	ND
Spirotetramat	0.02 / 0.06	13	N/A	ND
Tebuconazole	0.02 / 0.07	2	N/A	ND
Thiamethoxam	0.03 / 0.10	4.5	N/A	ND
Trifloxystrobin	0.03 / 0.08	30	N/A	ND

Mycotoxin Analysis

Mycotoxin analysis utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS).

Method: QSP 1212 - Analysis of Pesticides and Mycotoxins by LC-MS

Mycotoxin Test Results

07/31/2021

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Compound	LOD/LOQ (μg/kg)	Action Limit (μg/kg)	Measurement Uncertainty (μg/kg)	Result (μg/kg)
Aflatoxin B1	2.0 / 6.0		N/A	ND
Aflatoxin B2	1.8 / 5.6		N/A	ND
Aflatoxin G1	1.0 / 3.1		N/A	ND
Aflatoxin G2	1.2 / 3.5		N/A	ND
Ochratoxin A	6.3 / 19.2	20	N/A	ND
Total Aflatoxin		20	±	ND

Residual Solvents Analysis

Residual Solvent analysis utilizing gas chromatography-mass spectrometry (GC-MS).

Method: QSP 1204 - Analysis of Residual Solvents by GC-MS

Residual Solvents Test Results

07/31/2021

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)
1,2-Dichloroethane	0.05 / 0.1	1	N/A	ND
Benzene	0.03 / 0.09	1	N/A	ND
Chloroform	0.1 / 0.2	1	N/A	ND
Dichloromethane (Methylene Chloride)	0.3 / 0.9	1	N/A	ND
Ethylene Oxide	0.3 / 0.8	1	N/A	ND
Trichloroethylene	0.1 / 0.3	1	N/A	ND
2-Propanol (Isopropyl Alcohol)	10 / 40	5000	N/A	ND
Acetone	20 / 50	5000	N/A	ND
Acetonitrile	2 / 7	410	N/A	ND
Ethanol	20 / 50	5000	N/A	ND
Ethyl Acetate	20 / 60	5000	N/A	ND
Ethyl Ether	20 / 50	5000	N/A	ND
Methanol	50 / 200	3000	N/A	ND
n-Butane	10 / 50	5000	N/A	ND
n-Heptane	20 / 60	5000	N/A	ND
n-Hexane	2 / 5	290	N/A	ND
n-Pentane	20 / 50	5000	N/A	ND
Propane	10 / 20	5000	N/A	ND
Toluene	7 / 21	890	N/A	ND
Total Xylenes	50 / 160	2170	N/A	ND

Heavy Metals Analysis

Heavy metal analysis utilizing inductively coupled plasma-mass spectrometry (ICP-MS).

Method: QSP 1160 - Analysis of Heavy Metals by ICP-MS

Heavy Metals Test Results

07/29/2021

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)
Arsenic	0.02 / 0.1	1.5	N/A	ND
Cadmium	0.02 / 0.05	0.5	N/A	ND
Lead	0.04 / 0.1	0.5	N/A	ND
Mercury	0.002 / 0.01	3	N/A	ND

Microbiology Analysis

Analysis conducted by polymerase chain reaction (PCR) and fluorescence detection of microbiological contaminants. And Analysis conducted by 3M^TM Petrifilm^TM and plate counts of microbiological contaminants.

Method: QSP 1221 - Analysis of Microbiological Contaminants and QSP 6794 - Plating with 3M^TM Petrifilm^TM

Microbiology Test Results (PCR)

07/29/2021

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Compound	Action Limit	Result (cfu/g)
Yersinia spp.	Detect	ND
Salmonella spp.	Detect	ND
Candida albicans	Not Detected in 1g	ND
Aspergillus niger	Detect	ND
Aspergillus flavus	Detect	ND
Campylobacter spp.	Detect	ND
Aspergillus terreus	Detect	ND
Aspergillus fumigatus	Detect	ND
Staphylococcus aureus	Not Detected in 1g	ND
Listeria monocytogenes	Detect	ND
Pseudomonas aeruginosa	Not Detected in 1g	ND
Bile-Tolerant Gram-Negative Bacteria	100	ND
Shiga toxin-producing Escherichia coli	Detect	ND

Microbiology Test Results (PLATING)

07/29/2021

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Compound	Action Limit (cfu/g)	Result (cfu/g)
Coliforms	100	ND
Escherichia coli	Not Detected in 1g	ND
Total Yeast and Mold	10	ND
Total Aerobic Bacteria	100	ND
Total Enterobacteriaceae	1000	ND

COA ID: 210729U001-001

These results relate only to the sample included on this report. This report shall not be reproduced, except in full, without written approval of the laboratory.

Sample Certification: Action Limits used in this report are a compilation of guidance from state regulatory agencies in all states except Alaska. Action limits for required tests are the lower of any conflicting state regulations.

Decision Rule: Statements of conformity (e.g. Pass/Fail) to specifications are made in this report without taking measurement uncertainty into account. Where statements of conformity are made in this report, the following decision rules are applied: PASS - Results within limits/specifications, FAIL - Results exceed limits/specifications.

References: limit of detection (LOD), limit of quantification (LOQ), not detected (ND), not tested (NT), μg/g = ppm, μg/kg = ppb, too numerous to count >250 cfu/plate (TNTC), colony-forming unit (cfu)