Root Brew

Certificate of Analysis

HEMP QUALITY ASSURANCE TEST

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Sample Name:

Root Brew

Beverage, Colorado Infused

Total THC 7.9165 mg/unit

Total CBD 8.9460 mg/unit

Sum Cannabinoids 17.0400 mg/unit

Total Cannabinoids 17.0400 mg/unit

Sample Information

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Sample Details

Sample ID:250419Q005

Batch Number:240301RB-2

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Cannabinoid Analysis - Summary

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Total THC: 7.9165 mg/unit

Total CBD: 8.9460 mg/unit

Sum of Cannabinoids: 17.0400 mg/unit

Total Cannabinoids: 17.0400 mg/unit

Density:1.0471 g/mL

Total THC/CBD is calculated using the following formulas to take into account the loss of a carboxyl group during the decarboxylation step:
Total THC = Δ⁹-THC + (THCa (0.877))
Total CBD = CBD + (CBDa (0.877))

Sum of Cannabinoids = Δ⁹-THC + THCa + CBD + CBDa + CBG + CBGa + THCV + THCVa + CBC + CBCa + CBDV + CBDVa + Δ⁸-THC + CBL + CBN

Total Cannabinoids = (Δ⁹-THC+0.877*THCa) + (CBD+0.877*CBDa) + (CBG+0.877*CBGa) + (THCV+0.877*THCVa) + (CBC+0.877*CBCa) + (CBDV+0.877*CBDVa) + Δ⁸-THC + CBL + CBN

Why are Sum of Cannabinoids and Total Cannabinoids calculated separately?

Sum of Cannabinoids: The concentration of each individual cannabinoid added together as they are, without accounting for decarboxylation. This measurement is of particular importance for certain cannabis products that are not intended to be heated prior to application or consumption.

Total Cannabinoids: The concentration of each acidic cannabinoid, multiplied by 0.877 to account for the loss of molecular mass that happens as it is converted to a neutral cannabinoid when heated. Then this calculated concentration is added to the concentrations of the corresponding neutral cannabinoid already present. This corrected data is of particular importance to both producers and consumers of products intended to be heated prior to application or consumption.

Safety Analysis - Summary

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Pesticides: Pass

Mycotoxins: Pass

Residual Solvents: Pass

Heavy Metals: Pass

Microbiology (PCR): Pass

Microbiology (Plating): Pass

View Complete Test Results:

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Cannabinoid Analysis

Tested

Tested by high-performance liquid chromatography with diode-array detection (HPLC-DAD).

Method: QSP 1157 - Analysis of Cannabinoids by HPLC-DAD

Summary

Total THC: 7.9165 mg/unit

(Δ⁹-THC+0.877*THCa)

Total CBD: 8.9460 mg/unit

(CBD+0.877*CBDa)

Total Cannabinoids: 17.0400 mg/unit

Total CBG: ND

Total CBG (CBG+0.877*CBGa)

Total THCV: ND

Total THCV (THCV+0.877*THCVa)

Total CBC: ND

Total CBC (CBC+0.877*CBCa)

Total CBDV: ND

Total CBDV (CBDV+0.877*CBDVa)

Learn more

The cannabis plant contains dozens of active compounds called cannabinoids. These compounds are the primary contributors to the psychoactive effects of cannabis.

Cannabinoid testing determines the potency of a sample to aid in dosage considerations.

Cannabinoid Test Results

04/21/2025

Result Views

Table Pie Chart

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Compound	LOD/LOQ (mg/mL)	Measurement Uncertainty (mg/mL)	Result (mg/mL)	Result (%)
Cannabidiol (CBD)	0.0003 / 0.0008	±0.00094	0.0252	0.00241
Δ9 Tetrahydrocannabinol (Δ9THC)	0.0001 / 0.0011	±0.00122	0.0223	0.00213
Cannabinol (CBN)	0.0001 / 0.0005	±0.00001	0.0005	0.00005
Cannabichromene (CBC)	0.0003 / 0.0008	N/A	ND	ND
Cannabigerol (CBG)	0.0001 / 0.0005	N/A	ND	ND
Cannabicyclol (CBL)	0.0002 / 0.0008	N/A	ND	ND
Cannabichromenic Acid (CBCa)	0.0001 / 0.0011	N/A	ND	ND
Cannabidivarin (CBDV)	0.0002 / 0.0009	N/A	ND	ND
Cannabidiolic Acid (CBDa)	0.0001 / 0.0020	N/A	ND	ND
Cannabigerolic Acid (CBGa)	0.0001 / 0.0005	N/A	ND	ND
Tetrahydrocannabivarin (THCV)	0.0002 / 0.0009	N/A	ND	ND
Tetrahydrocannabinolic Acid (THCa)	0.0001 / 0.0004	N/A	ND	ND
Cannabidivarinic Acid (CBDVa)	0.0001 / 0.0014	N/A	ND	ND
Tetrahydrocannabivarinic Acid (THCVa)	0.0001 / 0.0014	N/A	ND	ND
∆8 Tetrahydrocannabinol (Δ8THC)	0.0006 / 0.0015	N/A	ND	ND
Total THC		±0.00122	0.0223 mg/mL	0.00213%
SUM OF CANNABINOIDS			0.0480 mg/mL	0.00458%

Unit Mass:

355 milliliters

Serving Size:

177 milliliters

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Δ⁹-THC per Unit		7.9165 mg/unit
Δ⁹-THC per Serving		3.9471 mg/serving
Total THC per Unit		7.9165 mg/unit
Total THC Per Serving		3.9471 mg/serving
CBD per Unit		8.9460 mg/unit
CBD per Serving		4.4604 mg/serving
Total CBD per Unit		8.9460 mg/unit
Total CBD per Serving		4.4604 mg/serving
Sum of Cannabinoids per Unit		17.0400 mg/unit
Sum of Cannabinoids per Serving		8.4960 mg/serving
Total Cannabinoids per Unit		17.0400 mg/unit
Total Cannabinoids per Serving		8.4960 mg/serving

Density Test Result

1.0471 g/mL Tested 04/21/2025 Method: QSP 7870 - Sample Preparation

Learn more

The cannabis plant contains dozens of active compounds called cannabinoids. These compounds are the primary contributors to the psychoactive effects of cannabis.

Cannabinoid testing determines the potency of a sample to aid in dosage considerations.

Pesticide Analysis

Pass

Pesticide and plant growth regulator analysis utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS) or gas chromatography-mass spectrometry (GC-MS).

*GC-MS utilized where indicated.

‡Analytes part of our California Select Panel.

Method: QSP 1212 - Analysis of Pesticides and Mycotoxins by LC-MS or QSP 1213 - Analysis of Pesticides by GC-MS

Pesticide Test Results

04/24/2025

PASS

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)	Result
Abamectin	0.032 / 0.097	0.25	N/A	ND	Pass
Acephate	0.006 / 0.018	0.05	N/A	ND	Pass
Acequinocyl	0.009 / 0.027	≥ LOQ	N/A	ND	Pass
Acetamiprid	0.016 / 0.049	0.05	N/A	ND	Pass
Aldicarb	0.030 / 0.090	0.5	N/A	ND	Pass
Allethrin	0.030 / 0.092	0.1	N/A	ND	Pass
Atrazine	0.006 / 0.019	≥ LOQ	N/A	ND	Pass
Azadirachtin	0.082 / 0.248	0.5	N/A	ND	Pass
Azoxystrobin	0.003 / 0.009	0.01	N/A	ND	Pass
Benzovindiflupyr	0.003 / 0.009	0.01	N/A	ND	Pass
Bifenazate	0.003 / 0.009	0.01	N/A	ND	Pass
Bifenthrin	0.021 / 0.064	≥ LOQ	N/A	ND	Pass
Boscalid	0.003 / 0.009	0.01	N/A	ND	Pass
Buprofezin^‡	0.006 / 0.019	≥ LOQ	N/A	ND	Pass
Carbaryl	0.007 / 0.020	0.025	N/A	ND	Pass
Carbofuran	0.003 / 0.008	0.01	N/A	ND	Pass
Chlorantraniliprole	0.006 / 0.018	≥ LOQ	N/A	ND	Pass
Chlorfenapyr*	0.005 / 0.015	1.5	N/A	ND	Pass
Chlorpyrifos	0.013 / 0.039	0.5	N/A	ND	Pass
Clofentezine	0.003 / 0.009	0.01	N/A	ND	Pass
Clothianidin	0.008 / 0.025	0.025	N/A	ND	Pass
Coumaphos	0.003 / 0.010	0.01	N/A	ND	Pass
Cyantraniliprole	0.003 / 0.010	0.01	N/A	ND	Pass
Cyfluthrin	0.052 / 0.159	≥ LOQ	N/A	ND	Pass
Cypermethrin	0.051 / 0.153	≥ LOQ	N/A	ND	Pass
Cyprodinil^‡	0.003 / 0.008	0.01	N/A	ND	Pass
Daminozide	0.026 / 0.077	≥ LOQ	N/A	ND	Pass
Deltamethrin	0.059 / 0.180	≥ LOQ	N/A	ND	Pass
Diazinon	0.006 / 0.017	≥ LOQ	N/A	ND	Pass
Dichlorvos (DDVP)	0.012 / 0.038	0.05	N/A	ND	Pass
Dimethoate	0.003 / 0.009	0.01	N/A	ND	Pass
Dimethomorph	0.016 / 0.050	≥ LOQ	N/A	ND	Pass
Dinotefuran	0.010 / 0.030	0.05	N/A	ND	Pass
Diuron	0.013 / 0.040	≥ LOQ	N/A	ND	Pass
Dodemorph	0.012 / 0.035	≥ LOQ	N/A	ND	Pass
Endosulfan sulfate	0.016 / 0.048	2.5	N/A	ND	Pass
Endosulfan-α*	0.004 / 0.014	2.5	N/A	ND	Pass
Endosulfan-β*	0.006 / 0.019	2.5	N/A	ND	Pass
Ethoprophos	0.003 / 0.009	0.01	N/A	ND	Pass
Etofenprox	0.014 / 0.042	≥ LOQ	N/A	ND	Pass
Etoxazole	0.007 / 0.020	≥ LOQ	N/A	ND	Pass
Etridiazole*	0.002 / 0.005	0.15	N/A	ND	Pass
Fenhexamid	0.003 / 0.008	≥ LOQ	N/A	ND	Pass
Fenoxycarb	0.003 / 0.010	0.01	N/A	ND	Pass
Fenpyroximate	0.007 / 0.020	≥ LOQ	N/A	ND	Pass
Fensulfothion	0.003 / 0.010	0.01	N/A	ND	Pass
Fenthion	0.003 / 0.010	0.01	N/A	ND	Pass
Fenvalerate^‡	0.033 / 0.099	≥ LOQ	N/A	ND	Pass
Fipronil	0.003 / 0.010	0.01	N/A	ND	Pass
Flonicamid	0.007 / 0.022	0.025	N/A	ND	Pass
Fludioxonil	0.003 / 0.010	0.01	N/A	ND	Pass
Fluopyram^‡	0.003 / 0.009	0.01	N/A	ND	Pass
Hexythiazox	0.003 / 0.010	≥ LOQ	N/A	ND	Pass
Imazalil	0.003 / 0.009	0.01	N/A	ND	Pass
Imidacloprid	0.003 / 0.010	0.01	N/A	ND	Pass
Iprodione	0.077 / 0.233	0.5	N/A	ND	Pass
Kinoprene	0.077 / 0.233	1.25	N/A	ND	Pass
Kresoxim-methyl	0.006 / 0.019	0.15	N/A	ND	Pass
λ-Cyhalothrin	0.068 / 0.206	≥ LOQ	N/A	ND	Pass
Malathion	0.003 / 0.009	0.01	N/A	ND	Pass
Metalaxyl	0.003 / 0.010	0.01	N/A	ND	Pass
Methiocarb	0.003 / 0.008	0.01	N/A	ND	Pass
Methomyl	0.008 / 0.025	0.025	N/A	ND	Pass
Methoprene^‡	0.172 / 0.521	≥ LOQ	N/A	ND	Pass
Mevinphos	0.008 / 0.024	0.025	N/A	ND	Pass
MGK-264	0.015 / 0.047	≥ LOQ	N/A	ND	Pass
Myclobutanil	0.003 / 0.009	0.01	N/A	ND	Pass
Naled	0.021 / 0.064	≥ LOQ	N/A	ND	Pass
Novaluron	0.002 / 0.005	0.025	N/A	ND	Pass
Oxamyl	0.017 / 0.051	1.5	N/A	ND	Pass
Paclobutrazol	0.003 / 0.010	0.01	N/A	ND	Pass
Parathion-methyl	0.016 / 0.050	≥ LOQ	N/A	ND	Pass
Pentachloronitrobenzene (Quintozene)*	0.004 / 0.012	≥ LOQ	N/A	ND	Pass
Permethrin	0.056 / 0.168	≥ LOQ	N/A	ND	Pass
Phenothrin	0.016 / 0.047	≥ LOQ	N/A	ND	Pass
Phosmet	0.007 / 0.020	≥ LOQ	N/A	ND	Pass
Piperonyl Butoxide	0.010 / 0.029	1.25	N/A	ND	Pass
Pirimicarb	0.003 / 0.009	0.01	N/A	ND	Pass
Prallethrin	0.015 / 0.046	≥ LOQ	N/A	ND	Pass
Propiconazole	0.027 / 0.080	≥ LOQ	N/A	ND	Pass
Propoxur	0.003 / 0.008	0.01	N/A	ND	Pass
Pyraclostrobin	0.003 / 0.010	0.01	N/A	ND	Pass
Pyrethrins	0.016 / 0.049	≥ LOQ	N/A	ND	Pass
Pyridaben	0.005 / 0.017	0.02	N/A	ND	Pass
Pyriproxyfen	0.003 / 0.009	≥ LOQ	N/A	ND	Pass
Resmethrin	0.013 / 0.039	0.05	N/A	ND	Pass
Spinetoram	0.003 / 0.010	0.01	N/A	ND	Pass
Spinosad	0.003 / 0.010	0.01	N/A	ND	Pass
Spirodiclofen	0.031 / 0.093	≥ LOQ	N/A	ND	Pass
Spiromesifen	0.016 / 0.050	≥ LOQ	N/A	ND	Pass
Spirotetramat	0.003 / 0.010	0.01	N/A	ND	Pass
Spiroxamine	0.020 / 0.062	≥ LOQ	N/A	ND	Pass
Tebuconazole	0.003 / 0.010	0.01	N/A	ND	Pass
Tebufenozide	0.003 / 0.008	0.01	N/A	ND	Pass
Teflubenzuron	0.007 / 0.022	0.025	N/A	ND	Pass
Tetrachlorvinphos	0.003 / 0.008	0.01	N/A	ND	Pass
Tetramethrin	0.021 / 0.063	≥ LOQ	N/A	ND	Pass
Thiabendazole	0.006 / 0.020	≥ LOQ	N/A	ND	Pass
Thiacloprid	0.003 / 0.009	0.01	N/A	ND	Pass
Thiamethoxam	0.003 / 0.010	0.01	N/A	ND	Pass
Thiophanate-methyl	0.013 / 0.040	≥ LOQ	N/A	ND	Pass
Trifloxystrobin	0.003 / 0.009	0.01	N/A	ND	Pass

Mycotoxin Analysis

Pass

Mycotoxin analysis utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS).

Method: QSP 1212 - Analysis of Pesticides and Mycotoxins by LC-MS

Mycotoxin Test Results

04/24/2025

PASS

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Compound	LOD/LOQ (μg/kg)	Action Limit (μg/kg)	Measurement Uncertainty (μg/kg)	Result (μg/kg)	Result
Aflatoxin B1	1.6 / 5.0	5	N/A	ND	Pass
Aflatoxin B2	1.4 / 4.1		N/A	ND
Aflatoxin G1	1.6 / 4.9		N/A	ND
Aflatoxin G2	1.6 / 5.0		N/A	ND
Ochratoxin A	1.6 / 5.0	5	N/A	ND	Pass
Total Aflatoxin		20	±	ND	Pass

Residual Solvents Analysis

Pass

Residual Solvent analysis utilizing gas chromatography-mass spectrometry (GC-MS).

Method: QSP 1204 - Analysis of Residual Solvents by GC-MS

Residual Solvents Test Results

04/22/2025

PASS

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)	Result
Propane	0.234 / 0.781	1000	N/A	ND	Pass
2-Methylpropane (Isobutane)	0.052 / 0.173		N/A	ND
n-Butane	0.019 / 0.063		N/A	ND
Total Butanes		1000	±	0	Pass
n-Pentane	0.310 / 1.033	1000	N/A	ND	Pass
n-Hexane	0.110 / 0.366	60	N/A	ND	Pass
2,2-Dimethylpentane (Neoheptane)	0.493 / 1.642		N/A	ND
2,3-Dimethylpentane	1.009 / 3.365		N/A	ND
2,4-Dimethylpentane	0.737 / 2.458		N/A	ND
3,3-Dimethylpentane	0.198 / 0.660		N/A	ND
2,2,3-Trimethylbutane (Triptane)	0.521 / 1.738		N/A	ND
2-Methylhexane (Isoheptane)	0.610 / 2.034		N/A	ND
3-Methylhexane	0.235 / 0.785		N/A	ND
3-Ethylpentane	0.304 / 1.012		N/A	ND
n-Heptane	13.12 / 43.72		N/A	ND
Total Heptanes		1000	±	0	Pass
Benzene	0.089 / 0.295	2	N/A	ND	Pass
Toluene	0.115 / 0.382	180	N/A	ND	Pass
1,3-Dimethylbenzene (m-Xylene) / 1,4-Dimethylbenzene (p-Xylene)	0.451 / 1.502		N/A	ND
1,2-Dimethylbenzene (o-Xylene)	0.387 / 1.289		N/A	ND
Total Xylenes		430	±	0	Pass
Methanol	53.92 / 163.4	600	N/A	ND	Pass
Ethanol	8.984 / 27.23	1000	N/A	ND	Pass
2-Propanol (Isopropyl Alcohol)	8.421 / 25.52	1000	N/A	ND	Pass
Acetone	10.59 / 32.08	1000	N/A	ND	Pass
Ethyl Acetate	1.123 / 3.745	1000	N/A	ND	Pass

Total Butanes = n-Butane + 2-Methylpropane (Isobutane)

Total Heptanes = 2,2-Dimethylpentane (Neoheptane) + 2,3-Dimethylpentane + 2,4-Dimethylpentane + 3,3-Dimethylpentane + 2,2,3-Trimethylbutane (Triptane) + 2-Methylhexane (Isoheptane) + 3-Methylhexane + 3-Ethylpentane + n-Heptane

Total Xylenes = 1,2-Dimethylbenzene (o-Xylene) + 1,3-Dimethylbenzene (m-Xylene) / 1,4-Dimethylbenzene (p-Xylene)

Heavy Metals Analysis

Pass

Heavy metal analysis utilizing inductively coupled plasma-mass spectrometry (ICP-MS).

Method: QSP 1160 - Analysis of Heavy Metals by ICP-MS

Heavy Metals Test Results

04/20/2025

PASS

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Compound	LOD/LOQ (μg/g)	Action Limit (μg/g)	Measurement Uncertainty (μg/g)	Result (μg/g)	Result
Arsenic	0.02 / 0.1	1.5	N/A	ND	Pass
Cadmium	0.02 / 0.05	0.5	N/A	ND	Pass
Lead	0.04 / 0.1	0.5	N/A	ND	Pass
Mercury	0.002 / 0.01	1.5	N/A	ND	Pass

Microbiology Analysis

Pass

Analysis conducted by polymerase chain reaction (PCR) and fluorescence detection of microbiological contaminants. And Analysis conducted by 3M^TM Petrifilm^TM and plate counts of microbiological contaminants.

Method: QSP 1221 - Analysis of Microbiological Contaminants

Microbiology Test Results (PCR)

04/23/2025

PASS

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Compound	Action Limit	Result	Result
Salmonella spp.	Not Detected in 1g	ND	Pass
Shiga toxin-producing Escherichia coli	Not Detected in 1g	ND	Pass

Microbiology Test Results (PLATING)

04/23/2025

PASS

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Compound	Action Limit (cfu/g)	Result (cfu/g)	Result
Coliforms	100	ND	Pass
Total Yeast and Mold	1000	ND	Pass
Total Aerobic Bacteria	10000	ND	Pass

Notes

COA Notes:

Sample serving mass provided by client. Sample unit mass provided by client.

COA ID: 250419Q005-001

For quality assurance purposes. Not a Regulatory Hemp Lab Test Report. These results relate only to the sample included on this report. This report shall not be reproduced, except in full, without written approval of the laboratory.

Sample Certification: 6 CCR 1010-21 Colorado Wholesale Food, Industrial Hemp, and Shellfish Regulations; where applicable

Decision Rule: Statements of conformity (e.g. Pass/Fail) to specifications are made in this report without taking measurement uncertainty into account. Where statements of conformity are made in this report, the following decision rules are applied: PASS - Results within limits/specifications, FAIL - Results exceed limits/specifications.

References: limit of detection (LOD), limit of quantification (LOQ), not detected (ND), not tested (NT), μg/g = ppm, μg/kg = ppb, too numerous to count >250 cfu/plate (TNTC), colony-forming unit (cfu)